The Effectiveness of Derris elliptica (Wall.) Benth Root Extract Against Temephos-resistant Aedes aegypti Larvae

Derris elliptica (Wall.) Benth is a wild plant or weed in agricultural fields that was traditionally used for poisoning fish and plant pests. Several studies showed the low lethal concentration of Derris elliptica root extract to control the laboratory strain Aedes aegypti larvae. This study aimed to evaluate the effectiveness of Derris elliptica root extract against the temephos-resistant Aedes aegypti larvae from the Dengue endemic areas. This experimental study used post-test only control group design with five different concentrations of methanolic Derris elliptica root extract, namely 0.09%, 0.13%, 0.17%, 0.21%, and 0.25%, respectively. Twenty-five of F1 temephos-resistant of wild-caught Aedes aegypti larvae were subjected to each group of the five different concentrations, and two control groups with aquadest. The temephos-resistant strain of Aedes aegypti larvae was determined by bioassay test with temephos -0.02 ppm compound. Mortality rate and lethal concentration (LC50 and LC90) of the larvae were calculated after 24-h contact. Average of mortality rate in each concentration after 24-h contact with Derris elliptica root extract were 32.8%, 49.6%, 72.0%, 95.2%, and 100% with LC50 and LC90 were 1,600 and 2,040 ppm, respectively. The methanolic Derris elliptica root extract have larvacidal potential to control the temephos-resistant Aedes aegypti larvae. Further research to obtain secondary metabolites is needed.