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Coenzyme Q10 ameliorates the quality of mouse oocytes during in vitro culture

Authors :
Juri Yang
Seung Jin Han
Chan Hee Lee
Dong Hyun Sohn
Hye Min Kim
Min Kook Kang
Source :
Zygote. 30:249-257
Publication Year :
2021
Publisher :
Cambridge University Press (CUP), 2021.

Abstract

SummaryOxidative stress causes several diseases and dysfunctions in cells, including oocytes. Clearly, oxidative stress influences oocyte quality during in vitro maturation and fertilization. Here we tested the ability of coenzyme Q10 (CoQ10) to reduce reactive oxygen species (ROS) and improve mouse oocyte quality during in vitro culture. Treatment with 50 μM CoQ10 efficiently reduced ROS levels in oocytes cultured in vitro. The fertilizable form of an oocyte usually contains a cortical granule-free domain (CGFD). CoQ10 enhanced the ratio of CGFD–oocytes from 35% to 45%. However, the hardening of the zona pellucida in oocytes was not affected by CoQ10 treatment. The in vitro maturation capacity of oocytes, which was determined by the first polar body extrusion, was enhanced from 48.9% to 75.7% by the addition of CoQ10 to the culture medium. During the parthenogenesis process, the number of two-cell embryos was increased by CoQ10 from 43.5% to 67.3%. Additionally, treatment with CoQ10 increased the expression of Bcl2 and Sirt1 in cumulus cells. These results suggested that CoQ10 had a positive effect on ROS reduction, maturation rate and two-cell embryo formation in mouse oocyte culture.

Details

ISSN :
14698730 and 09671994
Volume :
30
Database :
OpenAIRE
Journal :
Zygote
Accession number :
edsair.doi...........35ec38054da84a3e81c94301afec76c0
Full Text :
https://doi.org/10.1017/s0967199421000617