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An efficient method for detection of recombinant proteins using a bacteriophytochrome chromophore binding domain from Deinococcus Radiodurans

Authors :
Tae-Ryong Hahn
Bum-Soo Hahn
Seong Hee Bhoo
Sang-Won Lee
Tae-Lim Kim
Joo-Mi Yoon
Man-Ho Cho
Source :
Journal of the Korean Society for Applied Biological Chemistry. 56:103-106
Publication Year :
2013
Publisher :
Springer Science and Business Media LLC, 2013.

Abstract

Bacteriophytochromes are composed of N-terminal region for chromophore binding and C-terminal domain for transmission of a light signal. In this study, the possibility of using the chromophore-binding domain from a Deinococcus radiodurans bacteriophytochrome as a protein tag to enable a rapid and simple detection method for the production of recombinant proteins were evaluated.

Details

ISSN :
2234344X and 17382203
Volume :
56
Database :
OpenAIRE
Journal :
Journal of the Korean Society for Applied Biological Chemistry
Accession number :
edsair.doi...........3677110c4c0d428880f3859e13a6d003
Full Text :
https://doi.org/10.1007/s13765-012-2225-z