Back to Search
Start Over
Inactivation of indicators and pathogens in cattle feedlot manures and compost as determined by molecular and culture assays
- Source :
- FEMS Microbiology Ecology. 77:200-210
- Publication Year :
- 2011
- Publisher :
- Oxford University Press (OUP), 2011.
-
Abstract
- Accurate and conservative information about pathogen inactivation rates is needed as the basis for safe manure management on beef cattle feedlots. The survival of indicators and pathogens in faecal pen manure, stockpiled manure and manure compost was measured with autochthonous indicator bacteria (Escherichia coli, Clostridium perfringens, enterococci, total coliforms) and pathogens (Listeria monocytogenes, Campylobacter jejuni) using culture and/or real-time quantitative PCR (qPCR) methods. Additionally, the manures were incubated at 20, 37, 50 and 60 °C in microcosms to quantify the persistence of autochthonous microorganisms and selected process performance surrogates (Clostridium sporogenes, green fluorescent protein-labelled E. coli and L. monocytogenes). Estimated qPCR cell counts indicated that up to four orders of magnitude more target cells were present compared with the culturable counts. Corresponding T(90) estimates were up to sixfold higher. This study demonstrates the benefits of nucleic acid-based quantification of pathogen inactivation in cattle manures and concludes that the concurrent analysis of microorganisms by molecular and culture methods provides complementary value.
- Subjects :
- Manure management
Ecology
Compost
Clostridium sporogenes
Indicator bacteria
Biology
engineering.material
Beef cattle
Clostridium perfringens
medicine.disease_cause
biology.organism_classification
Applied Microbiology and Biotechnology
Microbiology
Manure
Listeria monocytogenes
engineering
medicine
Food science
Subjects
Details
- ISSN :
- 01686496
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi...........3a1574fd2ce60e3d1c233c3284c07469