Genotyping by next generation sequencing v1

This collection describes a standard genotyping procedure using next generation sequencing (NGS) in the Hockemeyer lab. Collection overview Preparing of genomic DNA from in vitro cultured cells A. Preparing crude cell lysate directly from hPSCs culture B. Preparing crude cell lysate from dissociated cells C. Preparing cell lysate from FACS-sorted cells Amplifying the target genomic region by PCR Preparing samples for NGS CRISPResso analysis General notes: 1. Throughout these protocols, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.