A Rapid UPLC-PAD Fingerprint Analysis of Chrysanthemum morifolium Ramat Combined with Chemometrics Methods

A novel approach for fingerprint analysis of Chrysanthemum morifolium Ramat was developed by combining chemometrics methods such as similarity analysis, hierarchical cluster analysis, and principal component analysis with ultra-performance liquid chromatography. The chromatographic separation was accomplished by gradient elution with mobile phase consisting of acetonitrile and phosphate buffer in 10 min prior to conventional liquid chromatography which usually takes over 1 h. Good reproducibility, precision, and accuracy were obtained with relative standard deviations below 2.10 % of six typical components. Consistent results were obtained in accordance with their cultivated sources. The proposed method above was confirmed to be more rapid and suitable, thus providing important criteria for further quality control of C. morifolium Ramat.