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A Rapid UPLC-PAD Fingerprint Analysis of Chrysanthemum morifolium Ramat Combined with Chemometrics Methods
- Source :
- Food Analytical Methods. 7:197-204
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- A novel approach for fingerprint analysis of Chrysanthemum morifolium Ramat was developed by combining chemometrics methods such as similarity analysis, hierarchical cluster analysis, and principal component analysis with ultra-performance liquid chromatography. The chromatographic separation was accomplished by gradient elution with mobile phase consisting of acetonitrile and phosphate buffer in 10 min prior to conventional liquid chromatography which usually takes over 1 h. Good reproducibility, precision, and accuracy were obtained with relative standard deviations below 2.10 % of six typical components. Consistent results were obtained in accordance with their cultivated sources. The proposed method above was confirmed to be more rapid and suitable, thus providing important criteria for further quality control of C. morifolium Ramat.
- Subjects :
- Reproducibility
Chromatography
biology
Chemistry
Chrysanthemum morifolium
Phosphate buffered saline
Analytical chemistry
biology.organism_classification
Applied Microbiology and Biotechnology
High-performance liquid chromatography
Analytical Chemistry
Chemometrics
Similarity analysis
Fingerprint
Principal component analysis
Safety, Risk, Reliability and Quality
Safety Research
Food Science
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi...........3e4c7bdb457feeed96bd633df53e3b3a