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The effect of freezing on bacteria

Authors :
R. B. Haines
Source :
Proceedings of the Royal Society of London. Series B - Biological Sciences. 124:451-463
Publication Year :
1938
Publisher :
The Royal Society, 1938.

Abstract

It has been known for many years that cultures of bacteria can be cooled to very low temperatures without destroying all the cells present (see, for example, MacFadyen and Rowland 1900). More recently the preservation of bacteria and sera by rapid freezing and drying has come into general use. The fundamental changes accompanying these processes have not so far been investigated in much detail. This paper presents the results of experiments on the effects of cooling and freezing at various temperatures on a number of bacterial species, and an attempt to correlate the observed effects of freezing on viability with the effect of freezing on the cellular proteins of B. pyocyaneus [Pseudomonas aeruginosa] . Hypotheses previously put forward to explain the observed effects of low temperatures on living cells may be briefly summarized under three headings

Details

ISSN :
20539193
Volume :
124
Database :
OpenAIRE
Journal :
Proceedings of the Royal Society of London. Series B - Biological Sciences
Accession number :
edsair.doi...........4113958c89103e74ca92d913e2a162d2
Full Text :
https://doi.org/10.1098/rspb.1938.0005