Anther Culture of Potato: Method and Applications

Anther culture provides a tool to produce haploid lines from cultivated potato (Solanum tuberosum L.), which has a tetraploid (2n = 4x = 48) genome constitution. Shoot regeneration via direct embryogenesis in anther culture procedure is preferred to produce dihaploid (2n = 2x = 24) potato lines, which can be applied in breeding of potato varieties. The anther culture protocol described in the present chapter can be conducted not only in cultivated potato (S. tuberosum) but also in other genetically related potato species.