Synthesis and characterization of cyclic PNC-28 Peptide, residues 17 – 26 (ETFSDLWKLL), a binding domain of p53

The present study reports the synthesis of cyclic PNC-28 peptide with solid phase peptide synthesis method. In first step we synthesize linear PNC-28 Peptide and in second step we cyclize (N-to-C or head-to-tail cyclization) the linear PNC-28 peptide. The molecular formula of cyclic PNC-28 peptide is C64H88N12O16 and its m/z mass is ≈1233.64. Elemental analysis of cyclic PNC-28 is C, 59.99; H, 6.92; N, 13.12; O, 19.98. The characterization of LC-MS, CD, FT-IR, 1HNMR has been done to confirm the successful synthesis and cyclization of linear PNC-28 peptide