Back to Search Start Over

Pathophysiological and Histopathological Ailments in Asphyxial Cardiac Arrest Induced Ischemic Renal Injury

Authors :
Md Rashedunnabi Akanda
Weishun Tian
Source :
The Pakistan Veterinary Journal. 41:64-70
Publication Year :
2021
Publisher :
Pakistan Veterinary Journal, 2021.

Abstract

Cardiac arrest (CA) is a sudden interruption in the effective blood flow due to heart failure. The current research aimed to conduct the pathophysiological and histopathological analysis in the kidney in asphyxial cardiac arrest rat model. Cardiac arrest was induced by intravenous injection of vecuronium bromide (2 mg/kg), following stop of mechanical ventilation. Rats were kept on the CA condition for 5 minutes. After that, cardiopulmonary resuscitation (CPR) was done to achieve return of spontaneous circulation (ROSC) following intravenous injection of epinephrine bolus (0.005 mg/kg), sodium bicarbonate (1 mEq/kg) and turn on mechanical ventilation. Then Rats were sacrificed after cardiopulmonary resuscitation (CPR) following asphyxial CA at 6 hrs, 12 hrs, 1 day, 2 days, and 5 days. The intensity of renal injury measured by the serum levels of blood urea nitrogen (BUN), creatinine (Crtn). Moreover, Hematoxylin & eosin, and Periodic Acid Schiff staining in the kidney was done for evaluating the renal histopathological changes. Furthermore, COX-2 immunoreactivity and western analysis were performed in the kidney. Survival rate declined following ROSC compared to the sham group, it showed 80% at 6 hrs and decreased time-dependently to 8% at 5 days. In this study, serum BUN and Crtn levels and renal histopathological scores significantly increased after ROSC in CA. Moreover, COX-2 expression also increased after ROSC in comparison to the sham group with its peak level at 5 days following CA. Renal histological damage score and COX-2 expression were upregulated after ROSC following CA. These results direct that COX-2 takes part in the asphyxial CA-induced ischemic renal injury

Details

ISSN :
20747764 and 02538318
Volume :
41
Database :
OpenAIRE
Journal :
The Pakistan Veterinary Journal
Accession number :
edsair.doi...........43d821a98ebb0799f394b940d8f5dcbb
Full Text :
https://doi.org/10.29261/pakvetj/2020.080