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Crystallization And Preliminary X-Ray Studies Of L-(+)-2,3-Butanediol Dehydrogenase From Brevibacterium Saccharolyticum C-1012

Authors :
Toshiaki Kudo
Masami Kusunoki
Masato Otagiri
Moriya Ohkum
Genji Kurisu
Sadaharu Ui
Source :
Protein & Peptide Letters. 8:57-61
Publication Year :
2001
Publisher :
Bentham Science Publishers Ltd., 2001.

Abstract

L(+)-2,3 -Butanediol dehydrogenase (L-BDH) from Brevibacterium saccharolyticum C-1012 has been crystallized by the hanging drop vapor diffusion method with polyethylene glycol 4000 as the precipitant. Crystals of L-BDH belong to the triclinic system, space group P1 with cell dimensions a = 60.8 A, b = 69.2 A, c = 127.4 A , alpha = 96.1 , beta = 100.2 and gama=109.6 . Crystals diffracted to 2.0 A resolution on a synchrotron radiation, and a full data set was collected at resolution of 2.0 A at 100K.c

Details

ISSN :
09298665
Volume :
8
Database :
OpenAIRE
Journal :
Protein & Peptide Letters
Accession number :
edsair.doi...........4435d75a2524a3e3242281d10c2a2059
Full Text :
https://doi.org/10.2174/0929866013409670