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Antioxidant capacity of egg, milk and soy protein hydrolysates and biopeptides produced by Bromelia pinguin and Bromelia karatas-derived proteases
- Source :
- Emirates Journal of Food and Agriculture. :122
- Publication Year :
- 2018
- Publisher :
- Faculty of Food and Agriculture, United Arab Emirates University, 2018.
-
Abstract
- Given the lack of knowledge in the generation and biological activity of biopeptides (BP) produced by Bromelia pinguin and Bromelia karatas-derived proteases, the objective of this work was to evaluate the antioxidant capacity (AOX) of protein hydrolysates and peptide fractions from hydrolysis of egg, milk and soy proteins with enzymes extracted from Bromelia pinguin and Bromelia karatas fruits. The degree of hydrolysis (DH) of the mentioned proteins was measured. The hydrolysates were sequentially fractionated by ultrafiltration using different membrane pore sizes (≤30, ≤10, ≤5 and ≤1 kDa) to obtain biopeptide fractions (BPF). The antioxidant capacity (AOX) of protein hydrolysates and BPF were evaluated. AOX was performed using 2,2´-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. after the enzymatic action of B. pinguin and B. karatas proteases, the highest DH values for egg, milk and soy protein hydrolysates ranged from 50-61%, 55-68% and 73-81%, respectively. The AOX was higher for soy hydrolysates than for egg and milk hydrolysates. Additionally, the highest AOX from ABTS and FRAP assays was observed for BPF ≤30 kDa, PF ≤5 kDa and ≤1 kDa, respectively. These results highlight the potential of B. pinguin and B. karatas proteases to hydrolyze food proteins and release bioactive peptides. Moreover, it has been shown that the hydrolysis products are a mixture of BP of different molecular weights, which could present AOX through different mechanisms.
- Subjects :
- 0106 biological sciences
Antioxidant
ABTS
Oxygen radical absorbance capacity
biology
010405 organic chemistry
Bromelia karatas
medicine.medical_treatment
biology.organism_classification
01 natural sciences
Applied Microbiology and Biotechnology
Hydrolysate
0104 chemical sciences
chemistry.chemical_compound
chemistry
010608 biotechnology
medicine
Bromelia
Animal Science and Zoology
Food science
Agronomy and Crop Science
Soy protein
Bromelia pinguin
Food Science
Subjects
Details
- ISSN :
- 20790538 and 2079052X
- Database :
- OpenAIRE
- Journal :
- Emirates Journal of Food and Agriculture
- Accession number :
- edsair.doi...........45373f92ee1daa9d03c8b82a0be5aa39