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H2S is involved in ABA-mediated stomatal movement through MPK4 to alleviate drought stress in Arabidopsis thaliana
- Source :
- Plant and Soil. 435:295-307
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Hydrogen sulfide (H2S) is a gaseous signaling molecule that participates in multiple physiological processes in both animals and plants. Mitogen-activated protein kinase (MAPK) is important signaling molecule that links the growth and developmental signals and environment stimuli to cellular responses. In the current study we explored the relationship between H2S and MAPK in drought stress resistance in Arabidopsis. The quantitative real-time (qRT)-PCR, root tip bending experiment and stomatal aperture assay were used in this paper. Drought stress activated both H2S biosynthesis and gene expression of MAPKs. The increase in MAPK expression was depressed in lcd/des1, a double mutant of H2S synthesis. Then we selected MPK4 as our target and used mpk4 mutants for further studies. H2S was able to alleviate the drought stress in wild-type (WT) Arabidopsis but not in mpk4 mutants. Meanwhile, H2S-induced stomatal movement was impaired in mpk4 mutants. We then examined the role of H2S and MPK4 in stomatal movements in response to abscisic acid (ABA) and hydrogen peroxide (H2O2). ABA- and H2O2- mediated stomatal movements were impaired in lcd/des1 and mpk4 mutants, and H2S-induced stomatal closure was impaired in slac1–3 mutants. Our results suggested that MPK4 is important downstream of H2S in the drought stress response and in stomatal movement, and that the H2S-MPK4 cascade is involved in ABA-mediated stomatal movement to regulate the drought stress.
- Subjects :
- 0106 biological sciences
MAPK/ERK pathway
biology
Kinase
fungi
Mutant
food and beverages
Soil Science
Plant physiology
04 agricultural and veterinary sciences
Plant Science
equipment and supplies
biology.organism_classification
01 natural sciences
Cell biology
chemistry.chemical_compound
chemistry
Arabidopsis
040103 agronomy & agriculture
0401 agriculture, forestry, and fisheries
Arabidopsis thaliana
Protein kinase A
Abscisic acid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15735036 and 0032079X
- Volume :
- 435
- Database :
- OpenAIRE
- Journal :
- Plant and Soil
- Accession number :
- edsair.doi...........4781773f9a3f682fb23cc0299dd2277c