PCR-DGGE Analysis of the Bacterial Community in Composting of Agriculture and Forestry Wastes with Different Microbial Agents

It is urgent for agricultural and forestry wastes (AFWS) to be handled efficiently as the generations of them are increasing rapidly in China. The paper studied the bacterial community variations in composting of AFWS with different three microbial agents by PCR-DGGE. Three microbial agents in the research werePhanerochaete chrysosporium(PC) ,Coriolus versicolor(CV) and GP (agent developed from laboratory with high lignocellulose degradation ability). DGGE profiles indicated that the populations of bacteria reduced from warming period to high-temperature phase fistly, then increased till cooling period, and finally descended during maturity period for all the composting treatments. The populations of bacteria in composting with GP innoculation keeped on a high level during high-temperature phase, cooling period and maturity period. In the early phase of the high temperature composting, Bacillus sp., Uncultured Bacillus sp., Pseudomonas sp., Uncultured Pseudomonas sp., Micrococcus sp., Brevibacterium, Sphingomonas sp., Aminobacter aminovorans and Uncultured Acinetobacter sp.were rich, and became the dominant flora of the period. The number of the same dominant flora in AFWS composting with GP and CV innoculations was the most during the high-temperature phase. Simultaneously, the number of the same dominant flora in AFWS composting with GP and PC innoculations was the most during cooling period. Therefore, GP has a good prospect in AFWS composting process for resource utilization.