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Identification of Cacao TIR-NBS-LRR Resistance Gene Homologues and Their Use as Genetic Markers
- Source :
- Journal of the American Society for Horticultural Science. 131:806-813
- Publication Year :
- 2006
- Publisher :
- American Society for Horticultural Science, 2006.
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Abstract
- Identifying genetic markers linked to disease resistance in plants is an important goal in marker-assisted selection. Using a candidate-gene approach, we have previously developed genetic markers in cacao (Theobroma cacao L.) for two families of genes involved in disease resistance: non-TIR-NBS-LRR (Toll/Interleukin-1 Receptor-nucleotide binding site-leucine rich repeat) resistance gene homologues and WRKY transcription factor genes; however, we failed to isolate TIR-NBS-LRR genes. Using a novel algorithm to design degenerate primers, we have now isolated TIR-NBS-LRR loci as determined by DNA sequence comparison. These loci have been developed as genetic markers using capillary array electrophoresis (CAE) and single-strand conformational polymorphism (SSCP) analysis. We have mapped three distinct TIR-NBS-LRR loci in an F2 population of cacao and demonstrated that one is located on linkage group 3 and the other two on linkage group 5.
Details
- ISSN :
- 23279788 and 00031062
- Volume :
- 131
- Database :
- OpenAIRE
- Journal :
- Journal of the American Society for Horticultural Science
- Accession number :
- edsair.doi...........47e584ee0df3e69f5a7073a23a9e820b