Back to Search
Start Over
Infection of non-host model plant species with the narrow-host-rangeCacao swollen shoot virus
- Source :
- Molecular Plant Pathology. 18:293-297
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Cacao swollen shoot virus (CSSV) is a major pathogen of cacao (Theobroma cacao) in Africa, and long-standing efforts to limit its spread by the culling of infected trees have had very limited success. CSSV is a particularly difficult virus to study, as it has a very narrow host range, limited to several tropical tree species. Furthermore, the virus is not mechanically transmissible, and its insect vector can only be used with difficulty. Thus, the only efficient means to infect cacao plants that have been experimentally described so far are by particle bombardment or the agroinoculation of cacao plants with an infectious clone. We have genetically transformed three non-host species with an infectious form of the CSSV genome: two experimental hosts widely used in plant virology (Nicotiana tabacum and N. benthamiana) and the model species Arabidopsis thaliana. In transformed plants of all three species, the CSSV genome was able to replicate, and, in tobacco, CSSV particles could be observed by immunosorbent electron microscopy, demonstrating that the complete virus cycle could be completed in a non-host plant. These results will greatly facilitate the preliminary testing of CSSV control strategies using plants that are easy to raise and to transform genetically.
- Subjects :
- 2. Zero hunger
0106 biological sciences
0301 basic medicine
biology
Theobroma
Nicotiana tabacum
fungi
food and beverages
Soil Science
Plant Virology
Plant Science
biology.organism_classification
01 natural sciences
Virology
Virus
Badnavirus
03 medical and health sciences
030104 developmental biology
Viral replication
Botany
Arabidopsis thaliana
Cacao swollen-shoot virus
Agronomy and Crop Science
Molecular Biology
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14646722
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Molecular Plant Pathology
- Accession number :
- edsair.doi...........47ea905ea37cb908ad231dfe5e891332