Possible therapeutic option of aqueous plasma for refractory ovarian cancer

Based on a number of recent reports, plasma exerts anti-proliferative and apoptotic effects on various cancer cells. Besides a direct effect on the cells, the proliferation of normal and tumorous mammalian cells was reported to be downregulated by indirect plasma-exposed medium through the generation of reactive oxidative species. We demonstrated that plasma-activated medium (PAM) also had an anti-tumor effect on even acquired chemoresistant OC cells. Furthermore, the aqueous plasma displayed an anti-tumor effect against clear-cell carcinoma, which is natively chemo-refractory OC, and we confirmed that the efficacy is selective for tumor cells rather than normal mesothelial cells. The adhesion potency to type-I-collagen-coated dishes was significantly lower in both chemosensitive and chemoresistant cells pretreated with PAM than that of non-stimulated cells (P