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Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA
- Source :
- Analytical Methods. 5:5005
- Publication Year :
- 2013
- Publisher :
- Royal Society of Chemistry (RSC), 2013.
-
Abstract
- A simple and label-free DNA electrochemical impedance biosensor has been developed for the sequence-specific recognition of double-stranded DNA (ds-DNA). The probe triplex-forming oligonucleotide (TFO) is immobilized on the gold electrode through Au–S interaction. In the presence of the target ds-DNA, the probe TFO interacted with the bases in the major groove of the ds-DNA to form a rigid triplex structure via Hoogsteen hydrogen bonds, leading to an increase of the electron-transfer resistance. It is found that the change of impedance has a linear relationship with the concentration of the target in the range of 0.1–40 nmol L−1, and the detection limit is as low as 0.04 nmol L−1 (S/N = 3).
Details
- ISSN :
- 17599679 and 17599660
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Analytical Methods
- Accession number :
- edsair.doi...........49a892ea3f3c9339c4004a307a892db1