Control of Growth and Development of Protocorm like Body Derived from Callus by Carbon Sources in Phalaenopsis

Protocorm like bodies (PLBs) derived from callus of Phalaenopsis utilized sucrose, maltose and sorbitol for their growth in vitro. These carbon sources affected differently and could control PLB growth. On sucrose supplemented medium a few PLBs produced plantlets and most others regenerated yellowish or greenish callus like body (CLB). Almost 80% of unrooted and 58% of rooted plantlets developed yellowish green CLB at the base of plantlets. On maltose supplemented medium, PLBs regenerated PLBs and a few plantlets. In subsequent culture, about 44% of unrooted and 24% of rooted plantlets initiated green PLBs at the base of cultured plantlets. On sorbitol supplemented medium, most of the PLBs developed plantlets and a few additional PLBs. Among the carbon sources tested, sorbitol supported plantlet development the best in vitro and proved to be the most suitable carbon source for plantlet initiation and development from PLB.