Back to Search
Start Over
Simple thiosemicarbazone 'switch' sensing of Hg2+ and biothiols in pure aqueous solutions and application to imaging in lysosomes
- Source :
- Journal of Molecular Structure. 1250:131811
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- A water-soluble salicylaldehyde-based thiosemicarbazone has been designed and applied to the selective fluorescent turn-off detection of Hg2+ ions. The probe-Hg2+ system acted as a turn-on probe toward biothiols, including cysteine, homocysteine, and glutathione. The limits of detection of the probe toward Hg2+, cysteine, homocysteine, and glutathione are 0.52 μM, 0.74 μM, 0.31 μM, and 0.27 μM, respectively. The mechanisms are carefully confirmed by fluorescence spectrometry, UV-visible spectrometry, electrospray ionization mass spectra, hydrogen nuclear magnetic resonance, time-resolved fluorescence spectrum, and density functional theory calculation. Moreover, due to the presence of a morpholine group, the probe was successfully used to monitor Hg2+ and biothiols in the lysosomes of HeLa cells.
Details
- ISSN :
- 00222860
- Volume :
- 1250
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Structure
- Accession number :
- edsair.doi...........4b0bf54ce8ba7c9a33934e799e2bdcfe