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Simultaneous detection of three antiviral and four antibiotic compounds in source-separated urine with liquid chromatography

Authors :
Tuula Tuhkanen
Sanna T. Pynnönen
Source :
Journal of Separation Science. 37:219-227
Publication Year :
2013
Publisher :
Wiley, 2013.

Abstract

An analytical method for the simultaneous screening of three antiviral agents (nevirapine, zidovudine, lamivudine), four antibiotics (sulfamethoxazole, trimethoprim, ciprofloxacin, rifampicin) and one reference compound (carbamazepine) in human urine was developed. Separation was achieved with a Kinetex XB-C18 (75 × 4.6 mm, 2.6 μm) column after the extraction of pharmaceuticals from urine with SPE. Gradient elution with a mobile phase consisting of acetonitrile and 10 mM KH2 PO4 (pH 2.5), and diode array detection with monitoring at 210 and 264 nm was applied. The developed method was validated in terms of selectivity, linearity, stability and sensitivity. Repeatability (n = 3) and between-day precision (n = 3) revealed RSD

Details

ISSN :
16159306
Volume :
37
Database :
OpenAIRE
Journal :
Journal of Separation Science
Accession number :
edsair.doi...........4b191dedb17971f117d20241eb3d7de5
Full Text :
https://doi.org/10.1002/jssc.201300492