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Delayed fluorescence, steady state fluorescence, photosystem II quantum yield as endpoints for toxicity evaluation of Cu2+ and Ag+
- Source :
- Environmental and Experimental Botany. 130:174-180
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The effect of copper and silver ions on photosynthetic parameters of the flagellate Euglena gracilis was determined. The photosystem II quantum yield (Y(PSII)) and steady state fluorescence (Ft) were measured with a PAM-fluorometer, while delayed fluorescence (DF) was determined by means of a sensitive single-photon camera attached to an image analysis device, which counted the relative number of photons released from the algae in darkness after previous light-stimulation. We found that after 3 h of incubation photosynthesis was affected only in the presence of relatively high concentrations of the used noble metals. The obtained EC50 values for Cu2+ were 505.8 mg L−1 (Y (PSII)), 286.5 mg L−1 (DF), and 491.0 mg L−1 (Ft). The EC50 values for silver ions were 51.9 mg L−1 (Y (PSII)), 51.8 mg L−1 (DF) (values for Ft not determinable). Photosynthesis is far less sensitive against toxic noble metals compared to motility parameters as reported in earlier studies, in part because the organelles of photosynthesis, the chloroplasts, are well protected from external substances within the cell, while flagella, reaching into the surrounding of the cell, are more easily influenced. As chloroplasts are easily accessible by means of non-invasive methods e.g. PAM- fluorometry, they are probably interesting model systems in order to detect effects of substances deep inside the cell. Influence on the chloroplasts is a measure that a germicide entered the cells, diffused through the cytoplasm and the membranous systems and reached compartmentalized enzyme complexes.
- Subjects :
- 0106 biological sciences
Euglena gracilis
Photosystem II
ved/biology
ved/biology.organism_classification_rank.species
Quantum yield
Plant Science
010501 environmental sciences
Biology
Photosynthesis
Photochemistry
01 natural sciences
Fluorescence
Fluorescence spectroscopy
Chloroplast
Cytoplasm
Agronomy and Crop Science
Ecology, Evolution, Behavior and Systematics
010606 plant biology & botany
0105 earth and related environmental sciences
Subjects
Details
- ISSN :
- 00988472
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Environmental and Experimental Botany
- Accession number :
- edsair.doi...........4d37dd5c39d0b2275f370b278ebf6286