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Bulk Acoustic Wave Biological Detection of Cefotaxime Sodium
- Source :
- Analytical Letters. 31:949-961
- Publication Year :
- 1998
- Publisher :
- Informa UK Limited, 1998.
-
Abstract
- A bulk acoustic wave (BAW) impedance sensor has been applied to rapid determination of in vitro concentrations of cefotaxime sodium. The method based on the growth frequency curve of Proteus Morganii, inhibited by cefotaxime sodium, is continuously monitored by using a BAW impedance sensor. The growth kinetic parameter, e.g. asymptote or maximum frequency shift (AF, Hz), estimated by fitting a modified Gompertz model to the growth frequency curve, is proportional to the logarithm of antibiotic concentration. The proposed method exhibits a favourable response to 0.25 – 20.0 μg mL−1 cefotaxime sodium with a detection limit of 0.03 μg mL−1, and the recoveries range from 97.3 to 107.5% (n=9). The results are in good agreement with those obtained by the conventional disk diffusion test (DDT) method. The effects of temperature and pH on the determination are discussed and in vitro susceptibility to cefotaxime of the genus Proteus has been investigated in detail. On leave from Department of Mathematics,...
- Subjects :
- Detection limit
Cefotaxime
Chemistry
Diffusion
Biochemistry (medical)
Clinical Biochemistry
Gompertz function
Analytical chemistry
Cefotaxime Sodium
Biochemistry
Analytical Chemistry
Electrochemistry
medicine
Asymptote
Quantitative analysis (chemistry)
Spectroscopy
medicine.drug
Antibacterial agent
Subjects
Details
- ISSN :
- 1532236X and 00032719
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Analytical Letters
- Accession number :
- edsair.doi...........4d3cd0497935230ff455589360b87710