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A facile and robust non-natural three enzyme biocatalytic cascade based on Escherichia coli surface assembly for fatty alcohol production
- Source :
- Energy Conversion and Management. 181:501-506
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Fatty alcohol is considered as a promising clean fuel alternative to fossil fuel. In this study, we developed a facile and robust multiple enzyme complex of lipase (LIP), carboxylic acid reductase (CAR) and aldehyde reductase (AHR) based on cohesin-dockerin specific interaction and E. coli-ice nucleation protein (INP) surface assembly for highly efficient conversion of triacylglycerols (TAGs) to fatty alcohols. The proximity effect, customized ratio and positional arrangement of the three enzymes were readily genetically generated. The optimized cell associated three enzyme complex gave 73% of conversion yield, and showed significantly improved stability in higher temperature, acidic/alkaline environments, polar organic solvents, high concentration of substrate, and good recyclability in successive biotransformations. The three enzyme complex provides an efficient non-natural synthetic cascade route for fatty alcohol bioproduction alternative to complex de novo biosynthesis route.
- Subjects :
- chemistry.chemical_classification
Enzyme complex
biology
Renewable Energy, Sustainability and the Environment
Chemistry
020209 energy
Energy Engineering and Power Technology
Substrate (chemistry)
Fatty alcohol
02 engineering and technology
Bioproduction
Combinatorial chemistry
chemistry.chemical_compound
Fuel Technology
Enzyme
020401 chemical engineering
Nuclear Energy and Engineering
Biosynthesis
0202 electrical engineering, electronic engineering, information engineering
biology.protein
0204 chemical engineering
Lipase
Aldehyde Reductase
Subjects
Details
- ISSN :
- 01968904
- Volume :
- 181
- Database :
- OpenAIRE
- Journal :
- Energy Conversion and Management
- Accession number :
- edsair.doi...........4d7d097454ad63433d04a9dcebce5212