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The Physicochemical Characteristics of Serum Albumin and Erythrocyte Cell Membranes under Normal and Heart Failure Symptom Conditions

Authors :
A. L. Dudylina
V. N. Titov
D. I. Grachev
Enno K. Ruuge
Source :
Biophysics. 64:721-728
Publication Year :
2019
Publisher :
Pleiades Publishing Ltd, 2019.

Abstract

—Spin labeling EPR spectroscopy was used to study the structural and conformational characteristics of human serum albumin and human erythrocyte membranes under normal conditions and with symptoms of heart failure. 5-Doxyl stearic acid and 16-doxyl stearic acid were used as spin labels, whose paramagnetic NO fragments are bound to different sites of the hydrocarbon chain. The EPR spectra of 16-doxyl stearic acid indicate that in the physiological temperature range serum albumin molecules are characterized by several types of fatty acid binding sites, which differ in parameters of spin-label rotational diffusion. This distribution of fatty-acid binding sites was typical for the blood serum of all patients who participated in our study, regardless of deviations from the normal blood parameters. The microviscosity of erythrocyte membranes from patient blood was measured using both 5-doxyl stearic and 16-doxyl stearic spin labels, whose paramagnetic fragments are located at different depths inside the lipid bilayer. It was found that in patients with an increased erythrocyte distribution width, the membrane lipid microviscosity is statistically significantly higher than under normal conditions.

Details

ISSN :
15556654 and 00063509
Volume :
64
Database :
OpenAIRE
Journal :
Biophysics
Accession number :
edsair.doi...........4f1123ccb384d65e6dbfecea3ca722df
Full Text :
https://doi.org/10.1134/s0006350919050051