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Initial purification of antimicrobial fermentation metabolites from Paecilomyces cicadae and its antimicrobial mechanism

Authors :
Lu-E Shi
Ming-Feng Xu
Zhen-Xing Tang
Ting Zhou
Yu Zhang
Da-Wei Xue
Xuelian Bai
Tao Chen
Qi-Wen Cen
Zheng-Yun Wang
Source :
LWT. 148:111785
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

In present study, the antimicrobial compounds (AMCs) from Paecilomyces cicadae (P. cicadae) were initially purified, and its antimicrobial mechanism was investigated. The results showed that the molecular weight of AMCs in crude protein extracts was below 10 kDa. The minimum inhibitory concentration (MIC) of crude protein extracts against Escherichia coli (E. coli) was 0.050 mg/mL. After E. coli was treated by crude protein extracts, we found that it could disrupt the structure of cell membrane, change the content of whole-cell or membrane proteins. In addition, crude protein extracts with certain concentrations also could effectively interact with bacterial DNA. Quantitative real-time PCR (qRT-PCR) results indicated that the expression levels of flagellar biosynthesis genes fliP, fliO, flgD and flhB were down-regulated, whereas dppF and dnaJ which are associated with the transport across the membrane and the interaction with dnaK, respectively, were up-regulated when E. coli was exposed to crude protein extracts. The present findings suggested that AMCs from P. cicadae showed a potential value for the development of antibacterial products.

Details

ISSN :
00236438
Volume :
148
Database :
OpenAIRE
Journal :
LWT
Accession number :
edsair.doi...........5007f1f781536df0808ce29cab2abcd9
Full Text :
https://doi.org/10.1016/j.lwt.2021.111785