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New SNPs and InDel Variations in SlMYB12 Associated with Regulation of Pink Color in Tomato
- Source :
- Tropical Plant Biology. 10:126-133
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Pink-fruited tomato is characterized by transparent peel with lack of flavonoid NC (Naringenin Chalcone). SlMYB12 is a transcription regulator of NC biosynthesis and marked as candidate gene regulating pink color trait of tomato. Causative allelic variations have been reported previously in the SlMYB12 as 603-bp deletion in promoter region, two SNPs (− > A) and (C > T) in the second exon and (G > T) nucleotide substitution in the second intron. Here, we tested these variations in eighty-five pink inbred lines and two red lines. Among them fifty lines were homozygous for the 603-bp deletion and twenty-eight for the insertion (− > A). No variations were observed in pink lines (16NWS01, 16NWS24, 16NWS28, 16NWS29, 16NWS30, 16NWS34 and 16NWS82). Cloning and sequencing of SlMYB12 gene revealed two new SNPs G > A at 1794 bp and 1851 bp positions of genomic sequence in six lines. While 44.75 Kb deletion was identified proximate to SlMYB12 region in the line 16NWS82. These new variations together with previously reported markers could serve as valuable markers in pink tomato breeding.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Candidate gene
Intron
food and beverages
Promoter
Plant Science
Biology
01 natural sciences
Molecular biology
03 medical and health sciences
Exon
030104 developmental biology
Genetic marker
Indel
Gene
Naringenin chalcone
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19359764 and 19359756
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Tropical Plant Biology
- Accession number :
- edsair.doi...........50fc2c7fed2143541fcbcd8b55e6f3c1