Immunoblotting of soluble antigens inParacoccidioides brasiliensisculture

Authors :: Eiko Nakagawa Itano
Nilson de Jesus Carlos
Mario Augusto Ono
Emerson José Venancio
Débora Alves Pinto
Ayako Sano
Zoilo Pires de Camargo
Source :: Microbiology and Immunology. 58:212-214
Publication Year :: 2014
Publisher :: Wiley, 2014.
Abstract: This study investigated the major soluble antigens produced by Paracoccidioides brasiliensis (Pb339) cultured in solid Sabouraud (pH 5.6 and 8.5), Sabouraud plus brain heart infusion and liquid tomato juice-enriched complex medium media at intervals of 3 days over 30 days by immunoblotting and concluded that, to optimize the source of each antigen, both time and growth conditions should be considered.