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Optimized signal detection and analysis methods for in vivo photoacoustic flow cytometry

Authors :
Quanyu Zhou
Qiyan Wang
Hao He
Ping Yang
Xiaoling Wang
Yuanzhen Suo
Wenyuan Gao
Shuo Tang
Zhenyu Niu
Xunbin Wei
Source :
SPIE Proceedings.
Publication Year :
2017
Publisher :
SPIE, 2017.

Abstract

Melanoma is known as a malignant tumor of melanocytes, which usually appear in the blood circulation at the metastasis stage of cancer. Thus the detection of circulating melanoma cells is useful for early diagnosis and therapy of cancer. Here we have developed an in vivo photoacoustic flow cytometry (PAFC) based on the photoacoustic effect to detect melanoma cells. However, the raw signals we obtain from the target cells contain noises such as environmental sonic noises and electronic noises. Therefore we apply correlation comparison and feature separation methods to the detection and verification of the in vivo signals. Due to similar shape and structure of cells, the photoacoustic signals usually have similar vibration mode. By analyzing the correlations and the signal features in time domain and frequency domain, we are able to provide a method for separating photoacoustic signals generated by target cells from background noises. The method introduced here has proved to optimize the signal acquisition and signal processing, which can improve the detection accuracy in PAFC.

Details

ISSN :
0277786X
Database :
OpenAIRE
Journal :
SPIE Proceedings
Accession number :
edsair.doi...........56aef39ddeba27cdff98bc79f67c8bd3
Full Text :
https://doi.org/10.1117/12.2253495