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Comparison of the local pulmonary distribution of nanoparticles administered intratracheally to rats via gavage needle or microsprayer delivery devices
- Source :
- Journal of Applied Toxicology. 37:502-507
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Intratracheal administration methods are used to conduct toxicological assessments of inhaled nanoparticles (NPs), and gavage needles or microsprayers are common intratracheal delivery devices. The NP suspension is delivered in a liquid state via gavage needle and as a liquid aerosol via microsprayer. The differences in local pulmonary NP distribution (called the microdistribution) arising from the different states of the NP suspension cause differential pulmonary responses; however, this has yet to be investigated. Herein, using microbeam X-ray fluorescence microscopy, we quantitatively evaluated the TiO2 pulmonary microdistribution (per mesh: 100 μm × 100 μm) in lung sections from rats administered an intratracheal dose of TiO2 NPs (6 mg kg−1) via gavage needle or microsprayer. The results revealed that: (i) using a microsprayer appears to reduce the variations in TiO2 content (ng mesh−1) among rats (e.g., coefficients of variation, n = 3, microsprayer vs gavage needle: 13% vs 30%, for the entire lungs); (ii) TiO2 appears to be deposited less in the right middle lobes than in the rest of the lung lobes, irrespective of the chosen intratracheal delivery device; and (iii) similar TiO2 contents (ng mesh−1) and frequencies are deposited in the lung lobes of rats administered TiO2 NPs via gavage needle or microsprayer. This suggests that the physical state of the administered NP suspension does not markedly alter TiO2 pulmonary microdistribution. The results of this investigation are important for the standardization of intratracheal administration methods. Copyright © 2016 John Wiley & Sons, Ltd.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Lung
Chemistry
Pharmacology
010403 inorganic & nuclear chemistry
Toxicology
01 natural sciences
0104 chemical sciences
Surgery
03 medical and health sciences
030104 developmental biology
medicine.anatomical_structure
Liquid state
medicine
Distribution (pharmacology)
Subjects
Details
- ISSN :
- 0260437X
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Toxicology
- Accession number :
- edsair.doi...........579e7a705988a7502faad579a14a338c