Wound Healing Properties of Collagen from the Bone of Two Marine Fishes

Type I collagen was extracted from the bone of two marine fishes; Magalaspis cordyla and Otolithes ruber and its efficacy in wound healing was studied. The yields of acid soluble (30.5 and 27.6%) and pepsin soluble (45.1 and 48.6%) collagen were respectively as per their dry weight basis. Extracted protein was characterized as type I collagen basing on SDS PAGE, UV–Vis, FTIR spectrometer and amino acid composition. The characterized collagen was cross-linked with glutaraldehyde and found to possess three dimensional pores and acted efficiently in healing the excision wounds made on Wistar rats. Bone is generally considered as waste and discarded in many processing plant. But, the current study proved that it has excellent medicinal importance and reveals a possible way to convert this waste into a medicinally important source.