Additional fie 1

Figure S1. qRT-PCR analysis of RANK-RANKL signaling-related gene expression to optimize the concentration of commercial sRANKL. The mRNA expressions of TRAF6, NFATc1, and TRAP were analyzed at day 6 after exposure of commercial sRANKL (20-50 ng/ml) to RAW 264.7 cells. The mRNA levels were normalized by GAPDH expression, and expressed as relative gene expression compared to control. For significance tests, a one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test were used, and expressed as follows; *P