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Technical note: Relationship between in situ NDF degradability and enzymatic NDF hydrolysis in forages, nonforage fibrous feeds, and crop residues1
- Source :
- Journal of Animal Science. 95:4172-4180
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- The study was performed on forages ( = 8), nonforage fibrous feeds ( = 10), and crop residues ( = 2). Samples were characterized for in situ NDF degradability (NDFD) at 6, 12, 18, 24, 30, 36, 48, 72, 96, 120, and 240 h of ruminal incubation. Then, samples were characterized for enzymatic NDFD by adopting a multistep enzymatic method consisting of a preincubation (PreInc) phase followed by enzymatic incubation (EnzInc) steps. In the PreInc phase, samples were incubated in a NaOH solution for 0, 30, 60, or 90 min. Then, in the EnzInc phase, samples were first incubated in a buffered enzymatic solution containing hemicellulase, cellulase, and Viscozyme L enzymes. Then, samples were incubated in a xylanase-buffered enzymatic solution. These 2-step EnzInc lasted for a total of 16 (8 h for the first enzymatic step + 8 h for the second enzymatic step), 32 (16 + 16 h), or 48 h (24 + 24 h). The enzymatic NDFD coefficients were increased by increasing both PreInc and EnzInc incubation times, and no PreInc × EnzInc interaction was observed, except for ryegrass hay. On average, enzymatic NDFD increased ( 0.80, < 0.05) and low errors of prediction were measured when specific enzymatic conditions were performed to predict in situ NDFD at intermediate (from 24 to 48 h) ruminal incubation. Generally, worse regression performances were obtained when enzymatic NDFD were used to predict in situ NDFD evaluated after shorter or longer incubation times. The direct prediction of the rate of NDF degradation was not possible using enzymatic NDFD coefficients. Even if the proposed multistep enzymatic method appeared promising, further studies are required to improve enzymatic NDFD prediction ability within specific forage types or nonforage fibrous feeds.
- Subjects :
- Crop residue
biology
0402 animal and dairy science
Forage
04 agricultural and veterinary sciences
General Medicine
Cellulase
040201 dairy & animal science
chemistry.chemical_compound
Hydrolysis
chemistry
Fodder
Biochemistry
Sodium hydroxide
040103 agronomy & agriculture
Genetics
Hay
biology.protein
0401 agriculture, forestry, and fisheries
Animal Science and Zoology
Food science
Incubation
Food Science
Subjects
Details
- ISSN :
- 15253163 and 00218812
- Volume :
- 95
- Database :
- OpenAIRE
- Journal :
- Journal of Animal Science
- Accession number :
- edsair.doi...........588db9f5753f0aeb94121310466bdd16
- Full Text :
- https://doi.org/10.2527/jas.2017.1630