Dispersion-induced behavior in sub-critical operation of a recombinant fed-batch fermentation with run-away plasmids

Fermentations with recombinant bacteria containing run-away plasmids are typically operated alternately above and below a critical temperature. To minimize the risks of run away reactions, it is preferable to keep the high temperature periods as short as possible. In this study the possibility of sustained low temperature (sub-critical) operation in a suitably non-homogeneous broth is analyzed. Fluid dispersion is used as a measure of non-homogeneity.