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Kinetic Analysis of HIF Prolyl Hydroxylases
Kinetic Analysis of HIF Prolyl Hydroxylases
- Source :
- Methods in Molecular Biology ISBN: 9781493976645
- Publication Year :
- 2018
- Publisher :
- Springer New York, 2018.
-
Abstract
- Kinetic analyses of HIF prolyl 4-hydroxylases (HIF-P4Hs) allow determination of substrate, cosubstrate and cofactor requirements, analysis of the reaction rate, and inhibitory properties of the isoenzymes in vitro. Here we describe an assay measuring the substrate hydroxylation-coupled decarboxylation of radioactive 2-oxoglutarate to radioactive carbon dioxide as a fast, efficient, and diverse method to analyze the enzyme kinetics of HIF-P4Hs.
Details
- ISBN :
- 978-1-4939-7664-5
- ISBNs :
- 9781493976645
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493976645
- Accession number :
- edsair.doi...........5a680b4838791937921d31da3116c708
- Full Text :
- https://doi.org/10.1007/978-1-4939-7665-2_2