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Kinetic Analysis of HIF Prolyl Hydroxylases

Kinetic Analysis of HIF Prolyl Hydroxylases

Authors :
Peppi Koivunen
Johanna Myllyharju
Source :
Methods in Molecular Biology ISBN: 9781493976645
Publication Year :
2018
Publisher :
Springer New York, 2018.

Abstract

Kinetic analyses of HIF prolyl 4-hydroxylases (HIF-P4Hs) allow determination of substrate, cosubstrate and cofactor requirements, analysis of the reaction rate, and inhibitory properties of the isoenzymes in vitro. Here we describe an assay measuring the substrate hydroxylation-coupled decarboxylation of radioactive 2-oxoglutarate to radioactive carbon dioxide as a fast, efficient, and diverse method to analyze the enzyme kinetics of HIF-P4Hs.

Details

ISBN :
978-1-4939-7664-5
ISBNs :
9781493976645
Database :
OpenAIRE
Journal :
Methods in Molecular Biology ISBN: 9781493976645
Accession number :
edsair.doi...........5a680b4838791937921d31da3116c708
Full Text :
https://doi.org/10.1007/978-1-4939-7665-2_2