Decreased Expression of PPARγ mRNA in Patients Correlates with aGVHD after Allo-HSCT

Background and objective: Peroxisome proliferator-activated receptor (PPAR)-gamma(γ) is a member of superfamily of nuclear hormone receptors and involved in the lipids metabolism, adipocyte differentiation, atherosclerosis and anti-inflammation. The role of PPARγ in acute graft versus host disease (aGVHD ) remains unclear. In this study, we aimed to investigate the role of PPARγ during aGVHD after allogenic hematopoietic stem cell transplantation (allo-HSCT ). Methods: 50 patients undering allo-HSCT and 20 healthy controls were enrolled in study. Peripheral blood (PB) of patients 30 days, 60 days, and 90 days were collected after allo-HSCT. We also collected PB samples at aGVHD onset and after aGVHD remission. Peripheral blood mononuclear cells (PBMCs) were isolated for real-time PCR to detect the mRNA expression of PPARγ, IFNγ, IL4, T-bet, GATA3, Foxp3, RORγt . Results: Expression of PPARγmRNA in healthy controls were significant lower than that in patients after allo-HSCT(P Conclusion: Our findings suggest that low expression of PPARγ is associated with aGVHD occurrence. PPARγ may be a useful indicator to predict aGVHD and follow-up. Disclosures No relevant conflicts of interest to declare.