Age-associated changes of oxidative modification and turnover of proteins

Possible involvement of active oxygen species has been shown in the generation of altered proteins accumulating in tissues of aged animals. Some discrepancies, however, appear to exist in age-associated changes in carbonyl content as a marker of oxidation of tissue proteins. Antibodies against oxidized proteins treated with dinitrophenyl hydrazine were prepared. The antibodies detected age- and tissue-specific patterns of carbonylated proteins by Western blot analysis.