Back to Search
Start Over
Ceramic Matrix for Large Scale Animal Cell Culture
- Source :
- Nature Biotechnology. 3:63-67
- Publication Year :
- 1985
- Publisher :
- Springer Science and Business Media LLC, 1985.
-
Abstract
- A ceramic matrix having a high degree of surface area per unit volume is shown to have significant utility in the large scale culture of animal cells. The surfaces of the ceramic provide for the adhesion and growth of a wide variety of cells to densities equal to or greater than obtained with other methods such as roller bottles or microcarriers. Utilizing an automated system controlling pH and dissolved oxygen, scale-up from 0.9 m2 to 18.5 m2 of surface area was accomplished with no losses in efficiency of surface utilization. The density of Vero cells after 7–8 days culture under standard conditions averaged 6.6 × 105 cells/cm2 for each size of ceramic. Methods providing continuous monitoring of the culture through analysis of the cellular oxygen consumption rate and complete cell harvesting are described.
- Subjects :
- Materials science
Scale (ratio)
Biomedical Engineering
Microcarrier
chemistry.chemical_element
Bioengineering
Adhesion
Ceramic matrix composite
Applied Microbiology and Biotechnology
Oxygen
Chemical engineering
chemistry
Cell culture
Cellular oxygen
visual_art
visual_art.visual_art_medium
Molecular Medicine
Ceramic
Biotechnology
Subjects
Details
- ISSN :
- 15461696 and 10870156
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Nature Biotechnology
- Accession number :
- edsair.doi...........5bc9501e115781d5d82f90a1393a5142