Study on Method of Extraction and Determination of Cucurbitin

Cucurbitin is a kind of specific alkaloids in pumpkin seed. The extraction conditions of cucurbitin were optimized as: extraction solvent was 4% dilute hydrochloric acid, rate of material to solvent was 1:20, ultrasonic power was 200 w and ultrasonic time was 75 min. The content of cucurbitin in pumpkin seed was determined by liquid chromatography. The linear between concentration and peak area were good within 160 μg mL-1. The recoveries were 96.50%~102.1%. The relative standard deviation was 1.08%. The results are accurate, true and reliable.