A sensitive chemiluminescence method for the determination of celecoxib in pharmaceutical and biological samples

A simple and sensitive chemiluminometric method was developed for the determination of celecoxib, a nonsteroidal anti-inflammatory drug. It is based on the sensitization of Ce(IV)-Na2SO3 chemiluminescence reaction by celecoxib in the presence of Tb(III) ions. The mechanism of chemiluminescence reaction was studied by obtaining fluorescence and chemiluminescence spectra. The effects of various chemical parameters on chemiluminescence intensity were investigated and optimized. Under the optimum conditions, a linear relationship was obtained between the enhanced chemiluminescence intensity and the concentration of celecoxib in the range of 0.01–0.15 μg/mL, with a limit of detection of 2.5 ng/mL. Study of interferences confirmed the selectivity of the developed method for analysis of pharmaceutical and biological samples. The method was applied to the determination of celecoxib in pharmaceutical formulations and human plasma samples, and also to dissolution studies with satisfactory results.