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HPTLC-Densitometry Determination of Riboflavin Fortified in Rice Noodle: Confirmed by SERS-Fingerprint

Authors :
Chen Yisheng
Bernhard Hellmann
Huang Caihong
Xueming Xu
Source :
Food Analytical Methods. 13:718-725
Publication Year :
2019
Publisher :
Springer Science and Business Media LLC, 2019.

Abstract

This paper presented a facile and efficient HPTLC method for quantifying riboflavin fortification in rice noodle that was a staple food popular in Asian countries. After high-throughput separation on silica gel plates with methanol/ethyl acetate/triethylamine (3/7/1, v/v/v) as the mobile phase, the obtained results were analyzed by fluorescence densitometry (mercury lamp, 360 nm excitation wavelength in combination with K400 optical filter). Quantification in this way offered high linearity (R2 = 0.9998 within 10–80 ng/band) and precision (RSD

Details

ISSN :
1936976X and 19369751
Volume :
13
Database :
OpenAIRE
Journal :
Food Analytical Methods
Accession number :
edsair.doi...........5d41be58cb5926f3fe24af8c4faeeccf
Full Text :
https://doi.org/10.1007/s12161-019-01694-2