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HPTLC-Densitometry Determination of Riboflavin Fortified in Rice Noodle: Confirmed by SERS-Fingerprint
- Source :
- Food Analytical Methods. 13:718-725
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- This paper presented a facile and efficient HPTLC method for quantifying riboflavin fortification in rice noodle that was a staple food popular in Asian countries. After high-throughput separation on silica gel plates with methanol/ethyl acetate/triethylamine (3/7/1, v/v/v) as the mobile phase, the obtained results were analyzed by fluorescence densitometry (mercury lamp, 360 nm excitation wavelength in combination with K400 optical filter). Quantification in this way offered high linearity (R2 = 0.9998 within 10–80 ng/band) and precision (RSD
- Subjects :
- Materials science
Chromatography
Silica gel
010401 analytical chemistry
Ethyl acetate
04 agricultural and veterinary sciences
040401 food science
01 natural sciences
Applied Microbiology and Biotechnology
Fluorescence
High-performance liquid chromatography
0104 chemical sciences
Analytical Chemistry
law.invention
Mercury-vapor lamp
chemistry.chemical_compound
Sodium borohydride
0404 agricultural biotechnology
chemistry
Fingerprint
law
Safety, Risk, Reliability and Quality
Densitometry
Safety Research
Food Science
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi...........5d41be58cb5926f3fe24af8c4faeeccf
- Full Text :
- https://doi.org/10.1007/s12161-019-01694-2