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Biosynthesis, Characterization and Biological Activities of Silver Nanoparticles from Pogostemon cablin Benth. Methanolic Leaf Extract
- Source :
- Journal of Nanoscience and Nanotechnology. 19:4109-4115
- Publication Year :
- 2019
- Publisher :
- American Scientific Publishers, 2019.
-
Abstract
- This study reports the biosynthesis of silver nanoparticles (AgNPs) using methanolic leaf extract of Pogostemon cablin Benth. (Patchouli) as a reducing agent, and their potent biological (antibacterial, antioxidant and anticancer) activities. The P. cablin extract when exposed to silver nitrate reduced silver ions to form crystalline AgNPs within 1 h of incubation at room-temperature. UV-visible spectra showed a sharp surface plasmon resonance (SPR) at around 430 nm for the biosynthesized AgNPs and the XRD pattern indicated the crystalline planes of the face centered cubic silver. The FE-SEM analysis revealed the occurrence of predominant spherical shaped AgNPs with a huge disparity in their particle size distribution with an average size of 25 nm, while, the FTIR data confirmed the bio-reduction and capping of AgNPs by several phytocompounds present in the methanolic leaf extract. AgNPs effectively inhibited the growth of all the tested human pathogenic bacterial strains (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli), while, the methanolic leaf extract failed to inhibit the growth of S. aureus and P. aeruginosa. AgNPs showed the highest free radical scavenging activity (79.0 ± 0.76%) compared to methanolic leaf extract (68.3 ± 0.68%) at 100 μg/ml. Further, the cytotoxicity study using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) confirmed that AgNPs successfully inhibited the human colon adenocarcinoma cell line (HT-29) in a dose dependent manner. At higher concentrations (500 μg/ml), only 4% of cells survived after 72 hrs of exposure with IC50 value of 120 μg/ml. Thus, these findings offer a new source of biomolecules with diverse biological activities.
- Subjects :
- Antioxidant
food.ingredient
Materials science
medicine.medical_treatment
Biomedical Engineering
Bioengineering
02 engineering and technology
Bacillus subtilis
medicine.disease_cause
Silver nanoparticle
chemistry.chemical_compound
food
medicine
General Materials Science
Escherichia coli
biology
General Chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
biology.organism_classification
Pogostemon
Silver nitrate
chemistry
Staphylococcus aureus
Patchouli
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 15334880
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of Nanoscience and Nanotechnology
- Accession number :
- edsair.doi...........5e8f69dfbb0f35d0e8f4953eed14dc42