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Metatranscriptomic insights of the metabolic process enhancement during food wastes fermentation driven by linear alkylbenzene sulphonates
- Source :
- Journal of Cleaner Production. 315:128145
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- In this study, the approach of stimulating the production of volatile fatty acids (VFAs) during food wastes (FW) fermentation by linear alkylbenzene sulphonates (LAS) is proposed and the underlying mechanisms from a metagenomic perspective are analyzed. The results show that with an appropriate level of LAS (50 mg/L), VFA production is greatly enhanced (11854 versus 1825 mg COD/L) with the increased proportions of high-value long-chain ones. Mechanistic explorations indicate that LAS contributes to the solubilization/hydrolysis of the main organic components in FW (i.e., carbohydrates, proteins and lipids) due to the amphipathic features. Besides, LAS would interact with the cell membranes and increase the cell permeability to promote the membrane transport of substrates into the functional microorganisms for further metabolisms. The metagenomic analysis demonstrates that the critical genes involved in hydrolase secretion (i.e., bglX, pepD and lip), ABC transporter (i.e., rfbA and lolD), main substrate metabolism (especially the proteins and lipids, i.e., glsA and glpK) and fatty acid biosynthesis (i.e., accA, accC and accD) all highly expressed in LAS-conditioned reactors. The upregulation of these encoding genes stimulates microbial metabolic activities and improves the production rates of VFAs. Besides, the distribution of functional microbial populations and their associations with genetic expressions in different reactors were investigated. This study provides profound information on the stimulating effects of LAS on FW fermentation for VFA production from genetic and functional perspectives.
- Subjects :
- Renewable Energy, Sustainability and the Environment
Chemistry
Linear alkylbenzene
020209 energy
Strategy and Management
Microorganism
05 social sciences
ATP-binding cassette transporter
02 engineering and technology
Building and Construction
Metabolism
Membrane transport
Industrial and Manufacturing Engineering
Hydrolysis
chemistry.chemical_compound
Biochemistry
050501 criminology
0202 electrical engineering, electronic engineering, information engineering
Fermentation
Metabolic Process
0505 law
General Environmental Science
Subjects
Details
- ISSN :
- 09596526
- Volume :
- 315
- Database :
- OpenAIRE
- Journal :
- Journal of Cleaner Production
- Accession number :
- edsair.doi...........5ee8092eb6be2f15593daf910e062d1b