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Overexpression of calmodulin gene fragment from Antarctic notothenioid fish improves chilling tolerance in Nicotiana benthamiana
- Source :
- Photosynthetica. 55:630-637
- Publication Year :
- 2017
- Publisher :
- Institute of Experimental Botany, 2017.
-
Abstract
- Calmodulin (CaM) is a highly conserved calcium sensor protein associated with chilling tolerance in living organisms. It has four EF-hand domains for binding of four Ca2+, two of them located in the N-terminus, and the other two in the C-terminus. A notothenioid CaM gene fragment (CaMm), which only codes for N-terminus of CaM (with two EF-hand domains), was introduced into Nicotiana benthamiana. Effects of its overexpression on chilling tolerance in plants were explored. During 4◦C or 0◦C chilling treatment, both CaMm and CaM transgenic plants showed higher PSII maximum quantum yield, actual quantum yield, and soluble protein content, lower electrolyte leakage and malondialdehyde content than that of the control. The changes in these physiological indices were comparable between the CaMm and CaM transgenic plants during the treatments. These results indicate that the N-terminus of calmodulin is likely the key functional domain involved in the adaptive response to cold stress.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Calmodulin
Physiology
fungi
food and beverages
Nicotiana benthamiana
Plant physiology
chemistry.chemical_element
Plant Science
Genetically modified crops
Calcium
biology.organism_classification
01 natural sciences
03 medical and health sciences
030104 developmental biology
Biochemistry
chemistry
Calcium-binding protein
biology.protein
Chlorophyll fluorescence
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15739058 and 03003604
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Photosynthetica
- Accession number :
- edsair.doi...........5fa8415094e15311e9c3113c15e3d941