AN IMPROVED SYNTHESIS OF PENTAPEPTIDE (SCAP1e) USING SOLID-PHASE METHOD WITH REDUCED RESIN LOADING TIME

SCAP1e is an alanine-rich-pentapeptide, analog of SCAP1 an antioxidant peptide. The previous solid-phase synthesis on this peptide resulted in low yield of the product (7%) due to the aggregation that might occur during synthesis. The aim of the research was to resynthesize this peptide using the similar method but with a slight modification in the resin loading step. SCAP1e was successfully synthesized through solid-phase method with an improved yield 87.9% and high purity (90.2%) based on the HPLC analysis. The reduction of resin loading value from 1.2 mmol/gram to 0.63 mmol/gram has made a good impact for the improvement of the synthetic yield.