Multiplexed Raman Imaging in Tissues and Living Organisms

Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are ideal multiplexing probes for in vivo imaging and tissue staining. Their remarkable sensitivity and unique Raman molecular fingerprint results in minimal background compared to other optical modalities. These characteristics also allow multiplexing down to the attomolar concentration. Here we describe the synthesis and in vivo multiplexing application of a SERS NP library.