New horizontal flow-through coil planet centrifuge for counter-current chromatography

The new type of horizontal flow-through coil planet centrifuge enables both preparative and analytical separations with partition efficiencies comparable to liquid chromatography. This counter-current chromatographic method was evaluated by the separation and purification of DNP-amino acids and various kinds of peptides using common two-phase solvent systems. The peptide samples separated include commercially available oligopeptides and two synthetic peptides of five and fifteen amino acids. A partition efficiency of up to several thousand theoretical plates was obtained with DNP-amino acids. Purification of the synthetic peptides, which produced problems with conventional liquid chromatography due to contamination and poor solubility, was successfully performed on a preparative scale with a reasonable degree of recovery.