Back to Search
Start Over
Hoechst-naphthalimide dyad with dual emissions as specific and ratiometric sensor for nucleus DNA damage
- Source :
- Chinese Chemical Letters. 28:2019-2022
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- A ratiometric fluorescent sensor (Hoe-NI) was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via “click chemistry”. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands (450 nm for Hoechst and 505 nm for naphthalimide) is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells.
- Subjects :
- DNA damage
Radical
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
Photochemistry
01 natural sciences
Fluorescence
0104 chemical sciences
Nuclear DNA
Fluorescence ratio
chemistry.chemical_compound
medicine.anatomical_structure
chemistry
Click chemistry
medicine
0210 nano-technology
Nucleus
DNA
Subjects
Details
- ISSN :
- 10018417
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Chinese Chemical Letters
- Accession number :
- edsair.doi...........64859a2571f6cb22efd564096fb9bd9c