Ozonation Degree of Vegetable Oils as the Factor of Their Anti-Inflammatory and Wound-Healing Effectiveness

The aim of this article is to study the in vivo effect of the ozonation degree of vegetal oils on two related clinical tests, such as inflammation and wound-healing in healthy and diabetic mice, respectively. Ozonated grape seed (GS) and sunflower (SF) oils were used for this purpose. The ozonation degree (from 0 to 44%) of oils was determined by the total unsaturation method (TU). This technique served to quantify the oxidizable substrate of oils and tissue’s lipids. For inflammation studies, an ear edema was induced by croton oil. The latter was treated with GS and SF oils with different ozonation degree, ozonated physiological solution and indomethacin, as reference. To evaluate the wound-healing effect, a circular section was surgically removed from the dorsal side of mice. The wound was treated with ozonated oils, Furacin® ointment (nitrofurazone 2%) and their combination. The TU of lipids (TUL) extracted from inflamed tissues was proposed to identify the biochemical changes in those tissues....