[26] General procedures for the induction and production of avian interferons

Publisher Summary This chapter discusses general procedures for the induction and production of avian interferons. Avian interferons may be induced directly in the allantoic fluid of the embryonated egg or in cell cultures produced from whole-embryo digests. The interferon-containing allantoic or culture fluid is then well suited for use as a crude preparation or in further purification schemes. For interferon production in cell cultures, embryo cell cultures are produced by using a standard tissue dissociation method. Newcastle disease virus (NDV) is inactivated by exposure to UV light. The virus is exposed just long enough completely to inactivate its ability to replicate, as monitored by inoculation into eggs or cell cultures. Cell cultures are inoculated with inactivated NDV at a ratio of 10 viral units per cell, determined by plaque assays done prior to inactivation.